Degradation of Edman

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Edman's Degradation - a method of sequencing peptides consisting in sequentially removing labeled amino acids from the N-terminus of a peptide molecule. Developed in 1950 by Swedish biochemist Pehra Edman. Summary reaction

Edman's degradation is done in two stages, alkaline and acidic. The first stage is the reaction of the N-phenyl isothiocyanate with the terminal amino group (-NH2), which in a mild alkaline medium is in an inert (unprotoned) form. The thiourea derivative thus obtained in the next step in the hydrochloric acid is cyclized and decomposed into a heterocyclic degradation product called the phenylthiohydantoin derivative of the amino acid (the so-called PTH-amino acid) and the peptide, one amino acid shorter. The resulting PTH-amino acids are identified by chromatography or electrophoresis. The mechanism of Edman's degradation

The maximum length of the sequenced peptide is 50-60 amino acids (due to the non-quantitative reaction). Edman sequencing can be performed in an automated way.

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